Drivers of older, more polluting vehicles will have to pay almost twice as much to drive in central London.
Mayor Sadiq Khan’s £10 T-Charge, which mainly applies to diesel and petrol vehicles registered before 2006, has come into force.
It covers the same area as the existing congestion charge zone, bumping up the cost to £21.50 for those affected.
Opponents said the scheme would “disproportionately penalise London’s poorest drivers”.
The measure is the latest attempt by Mr Khan to improve air quality in the capital and according to the mayor’s office, will impact 34,000 motorists a month.
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Mr Khan, said: “London’s air is a killer and is linked to asthma, strokes, heart disease and dementia.
“I refuse to be a mayor who ignores this and I am determined to take effective action to reduce the harm it does to Londoners.”
However, Simon Birkett, from the campaign group Clean Air London, said the move does not go far enough.
“The Mayor has pledged in his manifesto to restore London’s air quality to legal and safe limits and that means he has to do a whole lot more.
“We want him to take steps which are bigger, stronger an smarter.”
What is changing?
From Monday 23 October, there will be a £10 daily fee for those who drive more polluting vehicles in the congestion charging zone, on top of the existing £11.50 congestion charge.
Vehicles which do not comply with the Euro IV exhaust standard must pay the charge.
The standard defines emissions limits for cars, vans, buses, coaches and lorries. Most vehicles registered before 2006 are likely to exceed these limits.
The zone will operate between 07:00 and 18:00, Monday to Friday.
The T-Charge is the first of a series of new rates being introduced in London.
It is due to be replaced by a stricter Ultra-Low Emission Zone in 2020, although Mr Khan is consulting on bringing this forward to 2019.
This will mean diesel cars registered before September 2015 and petrol cars registered before 2006 will face a £12.50 charge.
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The mayor hopes to expand the area covered for cars and vans up to the North and South Circular roads in 2021.
City authorities in Birmingham, Leeds, Southampton, Derby and Nottingham have also been advised to impose charges for some polluting diesel vehicles by 2020, the Department for Environment, Food and Rural Affairs (Defra) said.
Paris, Grenoble and Lyon introduced an emission sticker scheme in January which splits vehicles into six different groups depending on their Euro Emissions standard.
Vehicles deemed too polluting – which includes petrol and diesel-powered cars registered before 1997 – are not granted a sticker, banning them from driving in the city during certain times.
Sue Terpilowski, from the Federation of Small Businesses, said: “The introduction of the T-Charge, comes at a time when small and micro-businesses in London are already facing astonishingly high property, employment and logistics costs.
“There is a fear that this will be the final straw that closes businesses and takes jobs.”
Shaun Bailey, conservative environment spokesman at the London Assembly, said: “As an asthmatic I’m well aware of how critical an issue this is for London but we need policies that actually deliver progress.
“By boasting about a policy that so disproportionately penalises London’s poorest drivers and puts jobs at risk, the mayor is simply blowing more smoke into the capital’s already-polluted atmosphere.”
Friends of the Earth air pollution campaigner Jenny Bates said: “Clearly the last thing individuals want is a new charge for moving around, but the grim reality is that nearly 10,000 early deaths are caused in London each year by the capital’s toxic air, so the Mayor is right to try to dissuade drivers bringing the oldest, dirtiest vehicles into central London.
“It’s only one small step towards clean air though – we urgently need a programme of meaningful financial assistance to help drivers of the dirtiest vehicles switch to something cleaner, and bold policies to cut traffic over all.”